| Congresso Brasileiro de Microbiologia 2023 | Resumo: 777-1 | ||||
Resumo:Biological modulators are known to improve gut microbiota and consequently maximize animal performance. Thus, the objective of the study was to evaluate the intestinal microbial ecology of broilers under the effect of a biological modulator. The experiment was conducted in the field on a poultry farm located in the State of Paraná, Brazil. The study was carried out in two sheds, with the litter used in Shed 1 as the negative control (T1) and the litter in Shed 2 as the treatment (T2), treated with the biological modulator BAC TRAT A®, both with first-use litter. Twenty samples of broiler feces were analyzed, referring to the two treatments (T1 and T2), with each treatment having ten replicates. To assess the microbial ecology of the samples, a 250-base segment of the V4 hypervariable region of the 16S rRNA ribosomal gene was amplified and subjected to polymerase chain reaction (PCR). The metagenomic library was constructed from these amplified samples, which were pooled and subsequently sequenced. Welch's test (P<0,05), followed by Bonferroni correction test, was used to compare bacterial genera between treatments. Taxonomic classification identified 228 bacterial genera. In T1, the most abundant genera were Bifidobacterium (22,56%), followed by Subdoligranulum (9,73%), Blautia (8,26%), and Lactobacillus (7,26%). Welch's test identified 33 genera with significantly different abundance (P<0,05) between the two treatments, including Faecalibacterium, Lactobacillus, Bifidobacterium, and Blautia. In the T2 group, a significant increase in the abundance of the genera Blautia, Alistipes, Roseburia, among others, was observed when compared to T1. The effect of the treatment on intestinal microbial ecology was evident, as the T2 group showed an increased amount of bacteria in the intestine that were positively associated with Faecalibacterium, a genus that has a positive relationship with zootechnical productivity. Based on the results obtained, we concluded that the use of modulators interfered with the findings of microbial ecology when comparing the control with the treatment, and this phenomenon can affect the productivity of broiler chicken. Palavras-chave: Poultry farm, Taxonomic classification, 16S rRNA ribosomal gene | |||||